{"id":6709,"date":"2024-02-21T06:49:51","date_gmt":"2024-02-21T06:49:51","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/hepatocytes-comprehensive-impedance-based-hepatotoxicity-assay-for-metabolically-active-ipsc-derived-hepatocytes\/"},"modified":"2024-02-22T16:23:18","modified_gmt":"2024-02-22T16:23:18","slug":"hepatocytes-comprehensive-impedance-based-hepatotoxicity-assay-for-metabolically-active-ipsc-derived-hepatocytes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/hepatocytes-comprehensive-impedance-based-hepatotoxicity-assay-for-metabolically-active-ipsc-derived-hepatocytes\/","title":{"rendered":"Hepatocytes &#8211; &#8220;Comprehensive impedance-based hepatotoxicity assay for metabolically active iPSC-derived hepatocytes&#8221;"},"content":{"rendered":"<p>Hepatotoxicity and drug-induced liver injury (DILI) are leading reasons for drug failure to market, leading to approximately 18% of market withdrawals of drugs in the last decade.<br \/>\nAdditionally, only half of drugs with a potential to induce hepatotoxicity are actually identified during preclinical animal studies. This highlights the importance of generating more<br \/>\nadvanced cell-based models and experimental strategies to enhance the predictivity of these assays. Current existing in vitro models employed to predict DILI mostly focus on<br \/>\nhepatocytes, though primary hepatocytes do not maintain their phenotype. iCell\u00ae Hepatocytes 2.0 (FUJIFILM Cellular Dynamics, FCDI) are human iPSC-derived cells with a<br \/>\nwide variety of basic and functional characteristics which make them amenable to applications such as compound mediated ADME-T and DILI toxicity. In addition to displaying<br \/>\ncharacteristic hepatocyte morphology, (i.e., polygonal shape, polynucleation and formation of bile canalicular channels), these cells also express liver cell markers, including albumin,<br \/>\nA1AT, and HNF4a, and exhibit basic and induced P450 functions, as observed in primary human hepatocytes. iCell\u00ae Hepatocytes 2.0 maintain morphology, marker expression,<br \/>\nand metabolic function in culture over a longer time frame compared to primary human hepatocytes, rendering these cells useful for investigations of acute and chronic DILI responses in a 2D culture system using impedance. Combining these cells with the planar gold-film electrodes on the impedance systems reveal alterations in confluency, cell contact (morphological shape) and conductivity of adherent cells, thereby providing a measure of toxicity. Dose-dependent harmful effects of drugs could be evaluated over time in a functional 2D cell-based model without the need for 3D spheroid formation.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[419,34],"resource_type":[90],"application_areas":[53,77],"therapeutic_areas":[290],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6709","resource_library","type-resource_library","status-publish","hentry","products_tags-atlaz","products_tags-cardioexcyte-96","resource_type-application-note","application_areas-assay-development-and-validation","application_areas-cell-ipscs-profiling","therapeutic_areas-toxicology"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6709","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6709"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6709"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6709"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6709"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6709"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6709"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6709"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6709"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6709"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}