{"id":6648,"date":"2023-02-17T07:15:21","date_gmt":"2023-02-17T07:15:21","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2022-pharmacological-dissection-of-the-crosstalk-between-nav-and-cav-channels-in-gh3b6-cells\/"},"modified":"2023-02-17T07:15:21","modified_gmt":"2023-02-17T07:15:21","slug":"2022-pharmacological-dissection-of-the-crosstalk-between-nav-and-cav-channels-in-gh3b6-cells","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2022-pharmacological-dissection-of-the-crosstalk-between-nav-and-cav-channels-in-gh3b6-cells\/","title":{"rendered":"2022 &#8211; Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells"},"content":{"rendered":"<p>Thanks to the crosstalk between Na<sup>+<\/sup> and Ca<sup>2+<\/sup> channels, Na<sup>+<\/sup> and Ca<sup>2+<\/sup> homeostasis interplay in so-called excitable cells enables the generation of action potential in response to electrical stimulation. Here, we investigated the impact of persistent activation of voltage-gated Na<sup>+<\/sup> (NaV) channels by neurotoxins, such as veratridine (VTD), on intracellular Ca<sup>2+<\/sup> concentration ([Ca<sup>2+<\/sup>]i) in a model of excitable cells, the rat pituitary GH3b6 cells, in order to identify the molecular actors involved in Na<sup>+<\/sup>-Ca<sup>2+<\/sup> homeostasis crosstalk. By combining RT-qPCR, immunoblotting, immunocytochemistry, and patch-clamp techniques, we showed that GH3b6 cells predominantly express the NaV1.3 channel subtype, which likely endorses their voltage-activated Na<sup>+<\/sup> currents. Notably, these Na<sup>+<\/sup> currents were blocked by ICA-121431 and activated by the \u03b2-scorpion toxin Tf2, two selective NaV1.3 channel ligands. Using Fura-2, we showed that VTD induced a [Ca<sup>2+<\/sup>]i increase. This effect was suppressed by the selective NaV channel blocker tetrodotoxin, as well by the selective L-type CaV channel (LTCC) blocker nifedipine. We also evidenced that crobenetine, a NaV channel blocker, abolished VTD-induced [Ca<sup>2+<\/sup>]i elevation, while it had no effects on LTCC. Altogether, our findings highlight a crosstalk between NaV and LTCC in GH3b6 cells, providing a new insight into the mode of action of neurotoxins.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61],"target_families":[332],"targets":[236],"resource_keyword":[],"class_list":["post-6648","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-publication","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_families-nav-channels","targets-nav1-3"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6648","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6648"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6648"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6648"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6648"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6648"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6648"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6648"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6648"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6648"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}