{"id":6575,"date":"2023-02-17T06:54:45","date_gmt":"2023-02-17T06:54:45","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2022-a-chip-based-array-for-high-resolution-fluorescence-characterization-of-free-standing-horizontal-lipid-membranes-under-voltage-clamp\/"},"modified":"2023-02-17T06:54:45","modified_gmt":"2023-02-17T06:54:45","slug":"2022-a-chip-based-array-for-high-resolution-fluorescence-characterization-of-free-standing-horizontal-lipid-membranes-under-voltage-clamp","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2022-a-chip-based-array-for-high-resolution-fluorescence-characterization-of-free-standing-horizontal-lipid-membranes-under-voltage-clamp\/","title":{"rendered":"2022 &#8211; A chip-based array for high-resolution fluorescence characterization of free-standing horizontal lipid membranes under voltage clamp"},"content":{"rendered":"<p>Optical techniques, such as fluorescence microscopy, are of great value in characterizing the structural dynamics of membranes and membrane proteins. A particular challenge is to combine high-resolution optical measurements with high-resolution voltage clamp electrical recordings providing direct information on e.g. single ion channel gating and\/or membrane capacitance. Here, we report on a novel chip-based array device which facilitates optical access with water or oil-immersion objectives of high numerical aperture to horizontal free-standing lipid membranes while controlling membrane voltage and recording currents using micropatterned Ag\/AgCl-electrodes. We demonstrate both wide-field and confocal imaging, as well as time-resolved single photon counting on free-standing membranes spanning sub-picoliter CaVities are demonstrated while electrical signals, including single channel activity, are simultaneously acquired. This optically addressable microelectrode CaVity array will allow combined electrical-optical studies of membranes and membrane proteins to be performed as a routine experiment.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[53],"therapeutic_areas":[],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6575","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6575","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6575"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6575"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6575"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6575"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6575"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6575"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6575"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6575"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6575"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}