{"id":6555,"date":"2023-02-17T07:04:17","date_gmt":"2023-02-17T07:04:17","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2021-study-of-anti-inflammatory-and-analgesic-activity-of-scorpion-toxins-dkk-sp1-2-from-scorpion-buthus-martensii-karsch-bmk\/"},"modified":"2023-02-17T07:04:18","modified_gmt":"2023-02-17T07:04:18","slug":"2021-study-of-anti-inflammatory-and-analgesic-activity-of-scorpion-toxins-dkk-sp1-2-from-scorpion-buthus-martensii-karsch-bmk","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2021-study-of-anti-inflammatory-and-analgesic-activity-of-scorpion-toxins-dkk-sp1-2-from-scorpion-buthus-martensii-karsch-bmk\/","title":{"rendered":"2021 &#8211; Study of Anti-Inflammatory and Analgesic Activity of Scorpion Toxins DKK-SP1\/2 from Scorpion Buthus martensii Karsch (BmK)"},"content":{"rendered":"<p>Buthus martensii\u00a0Karsch (BmK), is a kind of traditional Chinese medicine, which has been used for a long history for the treatment of many diseases, such as inflammation, pain and cancer. In this study, DKK-SP1\/2\/3 genes were screened and extracted from the cDNA library of\u00a0BmK.The DKK-SP1\/2\/3 were expressed by using plasmid pSYPU-1b in\u00a0E. coli\u00a0BL21, and recombinant proteins were obtained by column chromatography. In the xylene-induced mouse ear swelling and carrageenan-induced rat paw swelling model, DKK-SP1 exerted a significant anti-inflammatory effect by inhibiting the expression of NaV1.8 channel. Meanwhile, the release of pro-inflammatory cytokines(COX-2, IL-6) was decreased significantly and the release of anti-inflammatory cytokines (IL-10) were elevated significantly. Moreover, DKK-SP1 could significantly decrease the NaV1.8 current in acutelyisolated rat DRG neurons. In the acetic acid-writhing and ION-CCI model, DKK-SP2 displayed significant analgesic activity by inhibiting the expression of the NaV1.7 channel. Moreover, DKK-SP2 could significantly inhibit the NaV1.7 current in the hNaV1.7-CHO cells.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[56],"target_categories":[61],"target_families":[332],"targets":[117],"resource_keyword":[],"class_list":["post-6555","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-publication","application_areas-ion-channels","therapeutic_areas-pain","target_categories-voltage-gated-ion-channels","target_families-nav-channels","targets-nav1-8"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6555","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6555"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6555"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6555"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6555"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6555"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6555"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6555"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6555"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6555"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}