{"id":6495,"date":"2023-02-17T06:57:47","date_gmt":"2023-02-17T06:57:47","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2021-cell-engineering-method-using-fluorogenic-oligonucleotide-signaling-probes-and-flow-cytometry\/"},"modified":"2023-02-17T06:57:47","modified_gmt":"2023-02-17T06:57:47","slug":"2021-cell-engineering-method-using-fluorogenic-oligonucleotide-signaling-probes-and-flow-cytometry","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2021-cell-engineering-method-using-fluorogenic-oligonucleotide-signaling-probes-and-flow-cytometry\/","title":{"rendered":"2021 &#8211; Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry"},"content":{"rendered":"<p>Objective:\u00a0Chromovert\u00ae Technology is presented as a new cell engineering technology to detect and purify living cells based on gene expression.Methods:\u00a0The technology utilizes fluorogenic oligonucleotide signaling probes and flow cytometry to detect and isolate individual living cells expressing one or more transfected or endogenously-expressed genes.Results:\u00a0Results for production of cell lines expressing a diversity of ion channel and membrane proteins are presented, including heteromultimeric epithelial sodium channel (\u03b1\u03b2\u03b3-ENaC), sodium voltage-gated ion channel 1.7 (NaV1.7-\u03b1\u03b21\u03b22), four unique \u03b3-aminobutyric acid A (GABAA) receptor ion channel subunit combinations \u03b11\u03b23\u03b32s, \u03b12\u03b23\u03b32s, \u03b13\u03b23\u03b32s and \u03b15\u03b23\u03b32s, cystic fibrosis conductance regulator (CFTR), CFTR-\u0394508 and two G-protein coupled receptors (GPCRs) without reliance on leader sequences and\/or chaperones. In addition, three novel plasmid-encoded sequences used to introduce 3\u2032 untranslated RNA sequence tags in mRNA expression products and differentially-detectable fluorogenic probes directed to each are described. The tags and corresponding fluorogenic signaling probes streamline the process by enabling the multiplexed detection and isolation of cells expressing one or more genes without the need for gene-specific probes.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[75],"application_areas":[50,108],"therapeutic_areas":[109,254],"target_categories":[337,335,60],"target_families":[359,363,364],"targets":[110,190,192,215],"resource_keyword":[],"class_list":["post-6495","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-publication","application_areas-ion-channels","application_areas-transporters","therapeutic_areas-respiratory-diseases","therapeutic_areas-rare-diseases","target_categories-ligand-gated-ion-channels","target_categories-intracellular-regulated-ion-channels","target_categories-transporters","target_families-gabaa-receptors","target_families-cftr","target_families-atp-binding-cassette-abc-transporter","targets-cftr","targets-gabaa-1-2-2","targets-gabaa-5-3-2","targets-gabaa-1-3-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6495","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6495"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6495"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6495"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6495"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6495"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6495"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6495"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6495"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6495"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}