{"id":6426,"date":"2023-02-17T07:07:20","date_gmt":"2023-02-17T07:07:20","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2020-ionophore-constructed-from-non-covalent-assembly-of-a-g-quadruplex-and-liponucleoside-transports-k-ion-across-biological-membranes\/"},"modified":"2023-02-17T07:07:20","modified_gmt":"2023-02-17T07:07:20","slug":"2020-ionophore-constructed-from-non-covalent-assembly-of-a-g-quadruplex-and-liponucleoside-transports-k-ion-across-biological-membranes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2020-ionophore-constructed-from-non-covalent-assembly-of-a-g-quadruplex-and-liponucleoside-transports-k-ion-across-biological-membranes\/","title":{"rendered":"2020 &#8211; Ionophore constructed from non-covalent assembly of a G-quadruplex and liponucleoside transports K<sup>+<\/sup>-ion across biological membranes"},"content":{"rendered":"<p>The selective transport of ions across cell membranes, controlled by membrane proteins, is critical for a living organism. DNA-based systems have emerged as promising artificial ion transporters. However, the development of stable and selective artificial ion transporters remains a formidable task. We herein delineate the construction of an artificial ionophore using a telomeric DNA G-quadruplex (h-TELO) and a lipophilic guanosine (MG). MG stabilizes h-TELO by non-covalent interactions and, along with the lipophilic side chain, promotes the insertion of h-TELO within the hydrophobic lipid membrane. Fluorescence assays, electrophysiology measurements and molecular dynamics simulations reveal that MG\/h-TELO preferentially transports K<sup>+<\/sup>-ions in a stimuli-responsive manner. The preferential K<sup>+<\/sup>-ion transport is presumably due to conformational changes of the ionophore in response to different ions. Moreover, the ionophore transports K<sup>+<\/sup>-ions across CHO and K-562 cell membranes. This study may serve as a design principle to generate selective DNA-based artificial transporters for therapeutic applications.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6426","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-transporters","target_categories-transporters"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6426","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6426"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6426"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6426"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6426"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6426"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6426"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6426"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6426"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6426"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}