{"id":6371,"date":"2023-02-17T06:54:20","date_gmt":"2023-02-17T06:54:20","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2020-activation-of-the-archaeal-ion-channel-mthk-is-exquisitely-regulated-by-temperature\/"},"modified":"2023-02-17T06:54:21","modified_gmt":"2023-02-17T06:54:21","slug":"2020-activation-of-the-archaeal-ion-channel-mthk-is-exquisitely-regulated-by-temperature","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2020-activation-of-the-archaeal-ion-channel-mthk-is-exquisitely-regulated-by-temperature\/","title":{"rendered":"2020 &#8211; Activation of the archaeal ion channel MthK is exquisitely regulated by temperature"},"content":{"rendered":"<p>Physiological response to thermal stimuli in mammals is mediated by a structurally\u00a0diverse class of ion channels, many of which exhibit polymodal behavior. To probe the diversity of\u00a0biophysical mechanisms of temperature-sensitivity, we characterized the temperature-dependent\u00a0activation of MthK, a two transmembrane calcium-activated potassium channel from thermophilic\u00a0archaebacteria. Our functional complementation studies show that these channels are more\u00a0efficient at rescuing K<sup>+<\/sup> transport at 37\u00b0C than at 24\u00b0C. Electrophysiological activity of the purified\u00a0MthK is extremely sensitive (Q10 >100) to heating particularly at low-calcium concentrations\u00a0whereas channels lacking the calcium-sensing RCK domain are practically insensitive. By analyzing\u00a0single-channel activities at limiting calcium concentrations, we find that temperature alters the\u00a0coupling between the cytoplasmic RCK domains and the pore domain. These findings reveal a\u00a0hitherto unexplored mechanism of temperature-dependent regulation of ion channel gating and\u00a0shed light on ancient origins of temperature-sensitivity.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61,335],"target_families":[354],"targets":[],"resource_keyword":[],"class_list":["post-6371","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_categories-intracellular-regulated-ion-channels","target_families-calcium-and-sodium-activated-potassium-channels-kca-and-kna"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6371","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6371"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6371"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6371"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6371"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6371"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6371"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6371"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6371"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6371"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}