{"id":6369,"date":"2023-02-17T07:11:12","date_gmt":"2023-02-17T07:11:12","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2020-a-two-lane-mechanism-for-selective-biological-ammonium-transport\/"},"modified":"2023-02-17T07:11:13","modified_gmt":"2023-02-17T07:11:13","slug":"2020-a-two-lane-mechanism-for-selective-biological-ammonium-transport","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2020-a-two-lane-mechanism-for-selective-biological-ammonium-transport\/","title":{"rendered":"2020 &#8211; A two-lane mechanism for selective biological ammonium transport"},"content":{"rendered":"<p>The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt\/Mep\/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH4+\u00a0transport in two archetypal members of the family, the transporters AmtB from\u00a0Escherichia coli\u00a0and Rh50 from Nitrosomonas europaea. The pathway underpins a mechanism by which charged H+\u00a0and neutral NH3\u00a0are carried separately across the membrane after NH4+\u00a0deprotonation. This mechanism defines a new principle of achieving transport selectivity against competing ions in a biological transport process.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[374],"targets":[253],"resource_keyword":[],"class_list":["post-6369","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-uniporter","targets-amt"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6369","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6369"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6369"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6369"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6369"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6369"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6369"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6369"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6369"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6369"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}