{"id":6311,"date":"2023-02-17T07:22:46","date_gmt":"2023-02-17T07:22:46","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2019-fluorescent-artificial-receptor-based-membrane-assay-farma-for-spatiotemporally-resolved-monitoring-of-biomembrane-permeability\/"},"modified":"2023-02-17T07:22:46","modified_gmt":"2023-02-17T07:22:46","slug":"2019-fluorescent-artificial-receptor-based-membrane-assay-farma-for-spatiotemporally-resolved-monitoring-of-biomembrane-permeability","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2019-fluorescent-artificial-receptor-based-membrane-assay-farma-for-spatiotemporally-resolved-monitoring-of-biomembrane-permeability\/","title":{"rendered":"2019 &#8211; Fluorescent Artificial Receptor-Based Membrane Assay (FARMA) for Spatiotemporally Resolved Monitoring of Biomembrane Permeability"},"content":{"rendered":"<p>The spatiotemporally resolved monitoring of membrane translocation, e.g., of drugs or toxins, has been a long-standing goal. Herein, we introduce the fluorescent artificial receptor-based membrane assay (FARMA), a facile, label-free method. With FARMA, the permeation of more than hundred organic compounds (drugs, toxins, pesticides, neurotransmitters, peptides, etc.) through vesicular phospholipid bilayer membranes has been monitored in real time (\u00b5s-h time scale) and with high sensitivity (nM-\u00b5M concentration), affording permeability coefficients across an exceptionally large range from 10-9\u201110-3\u00a0cm s-1. From a fundamental point of view, FARMA constitutes a powerful tool to assess structure-permeability relationships and to test biophysical models for membrane passage. From an applied perspective, FARMA can be extended to high-throughput screening by adaption of the microplate reader format, to spatial monitoring of membrane permeation by microscopy imaging, and to the compartmentalized monitoring of enzymatic activity.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[129],"resource_type":[75],"application_areas":[53,99],"therapeutic_areas":[],"target_categories":[341],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6311","resource_library","type-resource_library","status-publish","hentry","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-assay-development-and-validation","application_areas-pores-and-toxins","target_categories-pores-and-toxins"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6311","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6311"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6311"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6311"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6311"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6311"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6311"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6311"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6311"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6311"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}