{"id":6309,"date":"2023-02-17T06:57:04","date_gmt":"2023-02-17T06:57:04","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2019-establishment-of-an-automated-patch-clamp-platform-for-electrophysiological-and-pharmacological-evaluation-of-hipsc-cms\/"},"modified":"2023-02-17T06:57:04","modified_gmt":"2023-02-17T06:57:04","slug":"2019-establishment-of-an-automated-patch-clamp-platform-for-electrophysiological-and-pharmacological-evaluation-of-hipsc-cms","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2019-establishment-of-an-automated-patch-clamp-platform-for-electrophysiological-and-pharmacological-evaluation-of-hipsc-cms\/","title":{"rendered":"2019 &#8211; Establishment of an automated patch-clamp platform for electrophysiological and pharmacological evaluation of hiPSC-CMs"},"content":{"rendered":"<p>Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology. However, the electrophysiological and pharmacological applications of hiPSC-CMs are hampered by manual patch-clamp technique, which is labor-intensive and generates a low-output. The automated patch-clamp technique is showing potential to overcome this problem. Here, we describe a new dissociation method, with which we can harvest a vast number of single relaxed hiPSC-CMs with smooth membrane suited for automated patch-clamp. Using the automated whole-cell patch-clamp technology, we report a high success rate for cell capture and whole-cell access (around 70%). We are able to identify and record several currents and paced action potentials (APs) with different success rates, including Na<sup>+<\/sup> current (INa), L-type Ca<sup>2+<\/sup> current (ICaL), two specific K<sup>+<\/sup> currents, the transient outward K<sup>+<\/sup> current (Ito) and the inward rectifier K<sup>+<\/sup> current (IK1). Moreover, we successfully applied dynamic current-clamp to virtually increase IK1 for AP recordings. Our study suggests that automated patch-clamp technology could be used to investigate the relevant ionic currents and APs in hiPSC-CMs. The combination of automated patch-clamp and hiPSC-CM technologies promises a wide range of applications in the future.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[75],"application_areas":[50,53,77],"therapeutic_areas":[54],"target_categories":[61],"target_families":[332,333,340],"targets":[91,93,96,285],"resource_keyword":[],"class_list":["post-6309","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","application_areas-cell-ipscs-profiling","therapeutic_areas-cardiac-safety-cipa","target_categories-voltage-gated-ion-channels","target_families-nav-channels","target_families-kv-channels","target_families-cav-channels","targets-cav1-2","targets-nav1-5","targets-kir2-1","targets-kv4-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6309","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6309"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6309"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6309"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6309"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6309"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6309"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6309"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6309"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6309"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}