{"id":6198,"date":"2023-02-17T07:11:06","date_gmt":"2023-02-17T07:11:06","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2017-mechanisms-of-charge-transfer-in-human-copper-atpases-atp7a-and-atp7b\/"},"modified":"2023-02-17T07:11:07","modified_gmt":"2023-02-17T07:11:07","slug":"2017-mechanisms-of-charge-transfer-in-human-copper-atpases-atp7a-and-atp7b","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2017-mechanisms-of-charge-transfer-in-human-copper-atpases-atp7a-and-atp7b\/","title":{"rendered":"2017 &#8211; Mechanisms of charge transfer in human copper ATPases ATP7A and ATP7B"},"content":{"rendered":"<p>ATP7A and ATP7B are Cu+\u00a0-transporting ATPases of subclass IB and play a fundamental role in intracellular copper homeostasis. ATP7A\/B transfer Cu+\u00a0ions across the membrane from delivery to acceptor proteins without establishing a free Cu+\u00a0gradient. Transfer of copper across the membrane is coupled to ATP hydrolysis. Current measurements on solid supported membranes (SSM) were performed to investigate the mechanism of copper-related charge transfer across ATP7A and ATP7B. SSM measurements demonstrated that electrogenic copper displacement occurs within ATP7A\/B following addition of ATP and formation of the phosphorylated intermediate. Comparison of the time constants for cation displacement in ATP7A\/B and sarcoplasmic reticulum Ca<sup>2+<\/sup>\u00a0-ATPase is consistent with the slower phosphoenzyme formation in copper ATPases. Moreover, ATP-dependent copper transfer in ATP7A\/B is not affected by varying the pH, suggesting that net proton counter-transport may not occur in copper ATPases. Platinum anticancer drugs activate ATP7A\/B and are subjected to ATP-dependent vectorial displacement with a mechanism analogous to that of copper.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[344],"targets":[207],"resource_keyword":[],"class_list":["post-6198","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-p-type-atpases","targets-serca"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6198","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6198"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6198"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6198"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6198"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6198"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6198"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6198"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6198"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6198"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}