{"id":6195,"date":"2023-02-17T07:22:10","date_gmt":"2023-02-17T07:22:10","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2017-ion-and-water-binding-sites-inside-an-occluded-hourglass-pore-of-a-trimeric-intracellular-cation-tric-channel\/"},"modified":"2023-02-17T07:22:11","modified_gmt":"2023-02-17T07:22:11","slug":"2017-ion-and-water-binding-sites-inside-an-occluded-hourglass-pore-of-a-trimeric-intracellular-cation-tric-channel","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2017-ion-and-water-binding-sites-inside-an-occluded-hourglass-pore-of-a-trimeric-intracellular-cation-tric-channel\/","title":{"rendered":"2017 &#8211; Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel"},"content":{"rendered":"<p>Background:Trimeric intracellular cation (TRIC) channels are crucial for Ca<sup>2+<\/sup>\u00a0handling in eukaryotes and are involved in K<sup>+<\/sup>\u00a0uptake in prokaryotes. Recent studies on the representative members of eukaryotic and prokaryotic TRIC channels demonstrated that they form homotrimeric units with the ion-conducting pores contained within each individual monomer.Results:Here we report detailed insights into the ion- and water-binding sites inside the pore of a TRIC channel from\u00a0Sulfolobus solfataricus\u00a0(SsTRIC). Like the mammalian TRIC channels,\u00a0SsTRIC is permeable to both K<sup>+<\/sup>\u00a0and Na<sup>+<\/sup>\u00a0with a slight preference for K<sup>+<\/sup>, and is nearly impermeable to Ca<sup>2+<\/sup>, Mg<sup>2+<\/sup>, or Cl\u2013. In the 2.2-\u00c5 resolution K<sup>+<\/sup>-bound structure of\u00a0SsTRIC, ion\/water densities have been well resolved inside the pore. At the central region, a filter-like structure is shaped by the kinks on the second and fifth transmembrane helices and two nearby phenylalanine residues. Below the filter, the cytoplasmic vestibule is occluded by a plug-like motif attached to an array of pore-lining charged residues.Conclusions:The asymmetric filter-like structure at the pore center of\u00a0SsTRIC might serve as the basis for the channel to bind and select monovalent cations. A Velcro-like plug-pore interacting model has been proposed and suggests a unified framework accounting for the gating mechanisms of prokaryotic and eukaryotic TRIC channels.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[129],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[335],"target_families":[373],"targets":[272],"resource_keyword":[],"class_list":["post-6195","resource_library","type-resource_library","status-publish","hentry","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-ion-channels","target_categories-intracellular-regulated-ion-channels","target_families-trimeric-intracellular-cation-channels","targets-tric-a-tric-b"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6195","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6195"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6195"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6195"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6195"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6195"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6195"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6195"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6195"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6195"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}