{"id":6162,"date":"2023-02-17T07:06:42","date_gmt":"2023-02-17T07:06:42","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2016-structural-and-functional-characterization-of-a-calcium-activated-cation-channel-from-tsukamurella-paurometabola\/"},"modified":"2023-02-17T07:06:42","modified_gmt":"2023-02-17T07:06:42","slug":"2016-structural-and-functional-characterization-of-a-calcium-activated-cation-channel-from-tsukamurella-paurometabola","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2016-structural-and-functional-characterization-of-a-calcium-activated-cation-channel-from-tsukamurella-paurometabola\/","title":{"rendered":"2016 &#8211; Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola"},"content":{"rendered":"<p>The selectivity filter is an essential functional element of K<sup>+<\/sup>\u00a0channels that is highly conserved both in terms of its primary sequence and its three-dimensional structure. Here, we investigate the properties of an ion channel from the Gram-positive bacterium\u00a0Tsukamurella paurometabola\u00a0with a selectivity filter formed by an uncommon proline-rich sequence. Electrophysiological recordings show that it is a non-selective cation channel and that its activity depends on Ca<sup>2+<\/sup>\u00a0concentration. In the crystal structure, the selectivity filter adopts a novel conformation with Ca<sup>2+<\/sup>\u00a0ions bound within the filter near the pore helix where they are coordinated by backbone oxygen atoms, a recurrent motif found in multiple proteins. The binding of Ca<sup>2+<\/sup>\u00a0ion in the selectivity filter controls the widening of the pore as shown in crystal structures and in molecular dynamics simulations. The structural, functional and computational data provide a characterization of this calcium-gated cationic channel.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61,338],"target_families":[350],"targets":[130,257],"resource_keyword":[],"class_list":["post-6162","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_categories-other-ion-channels","target_families-prokaryotic-tetrameric-cation-channels","targets-ph-gated-potassium-channel-kcsa","targets-nak"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6162"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6162"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6162"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6162"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6162"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6162"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6162"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6162"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}