{"id":6153,"date":"2023-02-17T07:10:57","date_gmt":"2023-02-17T07:10:57","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2016-membrane-pyrophosphatases-from-thermotoga-maritima-and-vigna-radiata-suggest-a-conserved-coupling-mechanism\/"},"modified":"2023-02-17T07:10:57","modified_gmt":"2023-02-17T07:10:57","slug":"2016-membrane-pyrophosphatases-from-thermotoga-maritima-and-vigna-radiata-suggest-a-conserved-coupling-mechanism","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2016-membrane-pyrophosphatases-from-thermotoga-maritima-and-vigna-radiata-suggest-a-conserved-coupling-mechanism\/","title":{"rendered":"2016 &#8211; Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism"},"content":{"rendered":"<p>Membrane-bound pyrophosphatases (M-PPases), which couple proton\/sodium ion transport to pyrophosphate synthesis\/hydrolysis, are important in abiotic stress resistance and in the infectivity of protozoan parasites. Here, three M-PPase structures in different catalytic states show that closure of the substrate-binding pocket by helices 5\u20136 affects helix 13 in the dimer interface and causes helix 12 to move down. This springs a \u2018molecular mousetrap\u2019, repositioning a conserved aspartate and activating the nucleophilic water. Corkscrew motion at helices 6 and 16 rearranges the key ionic gate residues and leads to ion pumping. The pumped ion is above the ion gate in one of the ion-bound structures, but below it in the other. Electrometric measurements show a single-turnover event with a non-hydrolysable inhibitor, supporting our model that ion pumping precedes hydrolysis. We propose a complete catalytic cycle for both proton and sodium-pumping M-PPases, and one that also explains the basis for ion specificity.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[344],"targets":[208],"resource_keyword":[],"class_list":["post-6153","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-p-type-atpases","targets-na-k-atpase"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6153","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6153"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6153"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6153"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6153"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6153"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6153"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6153"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6153"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6153"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}