{"id":6152,"date":"2023-02-17T06:51:40","date_gmt":"2023-02-17T06:51:40","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2016-mechano-pharmacological-characterization-of-cardiomyocytes-derived-from-human-induced-pluripotent-stem-cells\/"},"modified":"2023-02-17T06:51:40","modified_gmt":"2023-02-17T06:51:40","slug":"2016-mechano-pharmacological-characterization-of-cardiomyocytes-derived-from-human-induced-pluripotent-stem-cells","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2016-mechano-pharmacological-characterization-of-cardiomyocytes-derived-from-human-induced-pluripotent-stem-cells\/","title":{"rendered":"2016 &#8211; Mechano-Pharmacological Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells"},"content":{"rendered":"<p>Background\/ Aims:Common systems for the quantification of cellular contraction rely on\u00a0animal-based models, complex experimental setups or indirect approaches. The herein presented\u00a0CellDrum technology for testing mechanical tension of cellular monolayers and thin\u00a0tissue constructs has the potential to scale-up mechanical testing towards medium-throughput\u00a0analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug\u00a0testing and brings us closer to personalized drug medication.Methods:In the present study,\u00a0monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes\u00a0and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs\u00a0induced variations of the membrane deflection. The recorded contraction-relaxation-cycles\u00a0were analyzed with respect to their amplitudes, durations, time integrals and frequencies.\u00a0Besides unstimulated force and tensile stress, we investigated the effects of agonists and\u00a0antagonists acting on Ca<sup>2+<\/sup>\u00a0channels (S-Bay K8644\/verapamil) and Na<sup>+<\/sup> channels (veratridine\/\u00a0lidocaine).Results:The measured data and simulations for pharmacologically unstimulated\u00a0contraction resembled findings in native human heart tissue, while the pharmacological dose-response\u00a0curves were highly accurate and consistent with reference data.Conclusion:We\u00a0conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise\u00a0system for pharmacological, toxicological studies and offers new preclinical basic research\u00a0potential.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[35],"resource_type":[75],"application_areas":[53,76,77],"therapeutic_areas":[54],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6152","resource_library","type-resource_library","status-publish","hentry","products_tags-flexcyte-96","resource_type-publication","application_areas-assay-development-and-validation","application_areas-drug-discovery","application_areas-cell-ipscs-profiling","therapeutic_areas-cardiac-safety-cipa"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6152","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6152"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6152"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6152"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6152"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6152"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6152"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6152"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6152"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6152"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}