{"id":6143,"date":"2023-02-17T07:12:29","date_gmt":"2023-02-17T07:12:29","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2016-electrophysiological-characterization-of-the-archaeal-transporter-ncx_mj-using-solid-supported-membrane-technology\/"},"modified":"2023-02-17T07:12:29","modified_gmt":"2023-02-17T07:12:29","slug":"2016-electrophysiological-characterization-of-the-archaeal-transporter-ncx_mj-using-solid-supported-membrane-technology","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2016-electrophysiological-characterization-of-the-archaeal-transporter-ncx_mj-using-solid-supported-membrane-technology\/","title":{"rendered":"2016 &#8211; Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology"},"content":{"rendered":"<p>Sodium\u2013calcium exchangers (NCXs) are membrane transporters that play an important role in Ca<sup>2+<\/sup>\u00a0homeostasis and Ca<sup>2+<\/sup>\u00a0signaling. The recent crystal structure of NCX_Mj, a member of the NCX family from the archaebacterium\u00a0Methanococcus jannaschii, provided insight into the atomistic details of sodium\u2013calcium exchange. Here, we extend these findings by providing detailed functional data on purified NCX_Mj using solid supported membrane (SSM)\u2013based electrophysiology, a powerful but unexploited tool for functional studies of electrogenic transporter proteins. We show that NCX_Mj is highly selective for Na<sup>+<\/sup>, whereas Ca<sup>2+<\/sup>\u00a0can be replaced by Mg<sup>2+<\/sup>\u00a0and Sr2+\u00a0and that NCX_Mj can be inhibited by divalent ions, particularly Cd2+. By directly comparing the apparent affinities of Na<sup>+<\/sup>\u00a0and Ca<sup>2+<\/sup>\u00a0for NCX_Mj with those for human NCX1, we show excellent agreement, indicating a strong functional similarity between NCX_Mj and its eukaryotic isoforms. We also provide detailed instructions to facilitate the adaption of this method to other electrogenic transporter proteins. Our findings demonstrate that NCX_Mj can serve as a model for the NCX family and highlight several possible applications for SSM-based electrophysiology.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36,129],"resource_type":[75],"application_areas":[53,108],"therapeutic_areas":[],"target_categories":[60],"target_families":[343],"targets":[213,266],"resource_keyword":[],"class_list":["post-6143","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-assay-development-and-validation","application_areas-transporters","target_categories-transporters","target_families-exchanger","targets-ncx1-slc8a1","targets-ncx_mj"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6143","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6143"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6143"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6143"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6143"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6143"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6143"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6143"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6143"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6143"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}