{"id":6117,"date":"2023-02-17T06:53:02","date_gmt":"2023-02-17T06:53:02","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2015-automated-formation-of-lipid-membrane-microarrays-for-ionic-single-molecule-sensing-with-protein-nanopores\/"},"modified":"2023-02-17T06:53:03","modified_gmt":"2023-02-17T06:53:03","slug":"2015-automated-formation-of-lipid-membrane-microarrays-for-ionic-single-molecule-sensing-with-protein-nanopores","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2015-automated-formation-of-lipid-membrane-microarrays-for-ionic-single-molecule-sensing-with-protein-nanopores\/","title":{"rendered":"2015 &#8211; Automated Formation of Lipid Membrane Microarrays for Ionic Single-Molecule Sensing with Protein Nanopores"},"content":{"rendered":"<p>Efficient use of membrane protein nanopores in ionic single-molecule sensing requires technology for the reliable formation of suspended molecular membranes densely arrayed in formats that allow high-resolution electrical recording. Here, automated formation of bimolecular lipid layers is shown using a simple process where a poly(tetrafluoroethylene)-coated magnetic bar is remotely actuated to perform a turning motion, thereby spreading phospholipid in organic solvent on a nonpolar surface containing a <1 mm2\u00a04 \u00d7 4 array of apertures with embedded microelectrodes (microelectrode CaVity array). Parallel and high-resolution single-molecule detection by single nanopores is demonstrated on the resulting bilayer arrays, which are shown to form by a classical but very rapid self-assembly process. The technique provides a robust and scalable solution for the problem of reliable, automated formation of multiple independent lipid bilayers in a dense microarray format, while preserving the favorable electrical properties of the microelectrode CaVity array.\n<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[98],"resource_type":[75],"application_areas":[99],"therapeutic_areas":[],"target_categories":[341],"target_families":[347],"targets":[100,211],"resource_keyword":[],"class_list":["post-6117","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-16-tc","resource_type-publication","application_areas-pores-and-toxins","target_categories-pores-and-toxins","target_families-pore-forming-toxins-and-antibiotics","targets-alpha-hemolysin","targets-gramicidin"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6117","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6117"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6117"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6117"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6117"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6117"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6117"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6117"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6117"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}