{"id":6114,"date":"2023-02-17T07:10:45","date_gmt":"2023-02-17T07:10:45","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2015-a-sulfur%e2%80%90based-transport-pathway-in-cu%e2%80%90atpases\/"},"modified":"2023-02-17T07:10:45","modified_gmt":"2023-02-17T07:10:45","slug":"2015-a-sulfur%e2%80%90based-transport-pathway-in-cu%e2%80%90atpases","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2015-a-sulfur%e2%80%90based-transport-pathway-in-cu%e2%80%90atpases\/","title":{"rendered":"2015 &#8211; A sulfur\u2010based transport pathway in Cu+\u2010ATPases"},"content":{"rendered":"<p>Cells regulate copper levels tightly to balance the biogenesis and integrity of copper centers in vital enzymes against toxic levels of copper. PIB\u2010type Cu+\u2010ATPases play a central role in copper homeostasis by catalyzing the selective translocation of Cu+\u00a0across cellular membranes. Crystal structures of a copper\u2010free Cu+\u2010ATPase are available, but the mechanism of Cu+\u00a0recognition, binding, and translocation remains elusive. Through X\u2010ray absorption spectroscopy, ATPase activity assays, and charge transfer measurements on solid\u2010supported membranes using wild\u2010type and mutant forms of the Legionella pneumophila Cu+\u2010ATPase (LpCopA), we identify a sulfur\u2010lined metal transport pathway. Structural analysis indicates that Cu+\u00a0is bound at a high\u2010affinity transmembrane\u2010binding site in a trigonal\u2010planar coordination with the Cys residues of the conserved CPC motif of transmembrane segment 4 (C382 and C384) and the conserved Met residue of transmembrane segment 6 (M717 of the MXXXS motif). These residues are also essential for transport. Additionally, the studies indicate essential roles of other conserved intramembranous polar residues in facilitating copper binding to the high\u2010affinity site and subsequent release through the exit pathway.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[344],"targets":[207],"resource_keyword":[],"class_list":["post-6114","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-p-type-atpases","targets-serca"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6114","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6114"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6114"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6114"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6114"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6114"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6114"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6114"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6114"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6114"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}