{"id":6075,"date":"2023-02-17T07:02:16","date_gmt":"2023-02-17T07:02:16","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2013-functional-significance-of-thermosensitive-transient-receptor-potential-melastatin-channel-8-trpm8-expression-in-immortalized-human-corneal-endothelial-cells\/"},"modified":"2023-02-17T07:02:17","modified_gmt":"2023-02-17T07:02:17","slug":"2013-functional-significance-of-thermosensitive-transient-receptor-potential-melastatin-channel-8-trpm8-expression-in-immortalized-human-corneal-endothelial-cells","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2013-functional-significance-of-thermosensitive-transient-receptor-potential-melastatin-channel-8-trpm8-expression-in-immortalized-human-corneal-endothelial-cells\/","title":{"rendered":"2013 &#8211; Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells"},"content":{"rendered":"<p>Human corneal endothelial cells (HCEC) maintain appropriate tissue hydration and transparency by eliciting net ion transport coupled to fluid egress from the stroma into the anterior chamber. Such activity offsets tissue swelling caused by stromal imbibition of fluid. As corneal endothelial (HCE) transport function is modulated by temperature changes, we probed for thermosensitive transient receptor potential melastatin 8 (TRPM8) functional activity in immortalized human corneal endothelial cells (HCEC-12) and freshly isolated human corneal endothelial cells (HCEC) as a control. This channel is either activated upon lowering to 28\u00a0\u00b0C or by menthol, eucalyptol and icilin. RT-PCR and quantitative real-time PCR (qPCR) verified TRPM8 gene expression. Ca<sup>2+<\/sup>\u00a0transients induced by either menthol (500\u00a0\u03bcmol\/l), eucalyptol (3\u00a0mmol\/l), or icilin (2\u201360\u00a0\u03bcmol\/l) were identified using cell fluorescence imaging. The TRP channel blocker lanthanum III chloride (La3+, 100\u00a0\u03bcmol\/l) as well as the TRPM8 blockers BCTC (10\u00a0\u03bcmol\/l) and capsazepine (CPZ, 10\u00a0\u03bcmol\/l) suppressed icilin-induced Ca<sup>2+<\/sup>\u00a0increases. In and outward currents induced by application of menthol (500\u00a0\u03bcmol\/l) or icilin (50\u00a0\u03bcmol\/l) were detected using the planar patch-clamp technique. A thermal transition from room temperature to \u2248 18\u00a0\u00b0C led to Ca<sup>2+<\/sup>\u00a0increases that were inhibited by a TRPM8 blocker BCTC (10\u00a0\u03bcmol\/l). Other thermosensitive TRP pathways whose heterogeneous Ca<sup>2+<\/sup>\u00a0response patterns are suggestive of other Ca<sup>2+<\/sup>\u00a0handling pathways were also detected upon strong cooling (\u224810\u00a0\u00b0C). Taken together, functional TRPM8 expression in HCEC-12 and freshly dissociated HCEC suggests that HCE function can adapt to thermal variations through activation of this channel subtype.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[337,338],"target_families":[339],"targets":[122],"resource_keyword":[],"class_list":["post-6075","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-publication","application_areas-ion-channels","target_categories-ligand-gated-ion-channels","target_categories-other-ion-channels","target_families-trp-channels","targets-trpm8"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6075","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6075"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6075"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6075"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6075"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6075"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6075"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6075"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6075"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6075"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}