{"id":4811,"date":"2023-02-17T07:10:11","date_gmt":"2023-02-17T07:10:11","guid":{"rendered":"http:\/\/ionchanneltargets.com\/resource_library\/trpv4-activation-and-inhibition-of-trpv4-using-different-stimuli-on-the-port-a-patch\/"},"modified":"2023-02-17T07:10:11","modified_gmt":"2023-02-17T07:10:11","slug":"2010-solid-supported-membrane-technology-for-the-investigation-of-the-influenza-a-virus-m2-channel-activity","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2010-solid-supported-membrane-technology-for-the-investigation-of-the-influenza-a-virus-m2-channel-activity\/","title":{"rendered":"2010 &#8211; Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity"},"content":{"rendered":"<p>Influenza A virus encodes an integral membrane protein, A\/M2, that forms a pH-gated proton channel that is essential for viral replication. The A\/M2 channel is a target for the anti-influenza drug amantadine, although the effectiveness of this drug has been diminished by the appearance of naturally occurring point mutations in the channel pore. Thus, there is a great need to discover novel anti-influenza therapeutics, and, since the A\/M2 channel is a proven target, approaches are needed to screen for new classes of inhibitors for the A\/M2 channel. Prior in-depth studies of the activity and drug sensitivity of A\/M2 channels have employed labor-intensive electrophysiology techniques. In this study, we tested the validity of electrophysiological measurements with solid-supported membranes (SSM) as a less labor-intensive alternative technique for the investigation of A\/M2 ion channel properties and for drug screening. By comparing the SSM-based measurements of the activity and drug sensitivity of A\/M2 wild-type and mutant channels with measurements made with conventional electrophysiology methods, we show that SSM-based electrophysiology is an efficient and reliable tool for functional studies of the A\/M2 channel protein and for screening compounds for inhibitory activity against the channel.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-4811","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/4811","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=4811"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=4811"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=4811"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=4811"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=4811"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=4811"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=4811"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=4811"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=4811"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}