{"id":4787,"date":"2023-02-17T07:01:41","date_gmt":"2023-02-17T07:01:41","guid":{"rendered":"http:\/\/ionchanneltargets.com\/resource_library\/herg-characterization-of-herg-cho-on-nanions-port-a-patch\/"},"modified":"2023-02-17T07:01:42","modified_gmt":"2023-02-17T07:01:42","slug":"2010-characterization-of-two-pore-channel-2-tpcn2-mediated-ca2-currents-in-isolated-lysosomes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2010-characterization-of-two-pore-channel-2-tpcn2-mediated-ca2-currents-in-isolated-lysosomes\/","title":{"rendered":"2010 &#8211; Characterization of two pore channel 2 (TPCN2) -mediated Ca<sup>2+<\/sup> currents in isolated lysosomes"},"content":{"rendered":"<p>Two-pore channels (TPCNs) have been proposed to form lysosomal Ca<sup>2+<\/sup>\u00a0release channels that are activated by nicotinic acid adenine dinucleotide phosphate. Here, we employ a glass chip-based method to record for the first time nicotinic acid adenine dinucleotide phosphate -dependent currents through a two-pore channel (TPCN2) from intact lysosomes. We show that TPCN2 is a highly selective Ca<sup>2+<\/sup>\u00a0channel that is regulated by intralysosomal pH. Using site-directed mutagenesis, we identify an amino acid residue in the putative pore region that is crucial for conferring high Ca<sup>2+<\/sup>\u00a0selectivity. Our glass chip-based method will provide electrophysiological access not only to lysosomal TPCN channels but also to a broad range of other intracellular ion channels.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[75],"application_areas":[50,53],"therapeutic_areas":[],"target_categories":[61],"target_families":[370],"targets":[229],"resource_keyword":[],"class_list":["post-4787","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","target_categories-voltage-gated-ion-channels","target_families-two-pore-segment-channels-tpc","targets-tpc1-tpc2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/4787","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=4787"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=4787"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=4787"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=4787"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=4787"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=4787"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=4787"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=4787"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=4787"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}