{"id":19710,"date":"2026-07-28T12:38:33","date_gmt":"2026-07-28T10:38:33","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=19710"},"modified":"2026-07-28T12:38:33","modified_gmt":"2026-07-28T10:38:33","slug":"2026-cryo-em-insights-into-isoform-specific-properties-of-the-ip3r2-channel","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2026-cryo-em-insights-into-isoform-specific-properties-of-the-ip3r2-channel\/","title":{"rendered":"2026 &#8211; Cryo-EM insights into isoform-specific properties of the IP3R2 channel"},"content":{"rendered":"<p>Calcium release through inositol 1,4,5-trisphosphate receptors (IP<sub>3<\/sub>Rs) is a fundamental signaling mechanism that regulates diverse cellular processes. Among the three mammalian IP<sub>3<\/sub>R isoforms, IP<sub>3<\/sub>R2 is widely expressed, yet its structural basis for activation and regulation remains unclear. Here, we report cryo-EM structures of mammalian IP<sub>3<\/sub>R2 in ligand-free (closed) and Ca<sup>2+\/<\/sup>IP<sub>3<\/sub>\/ATP-bound (activated) states at 3.3\u2009\u00c5 and 3.6\u2009\u00c5 resolution, respectively. These structures define the architecture of IP<sub>3<\/sub>R2 and reveal conformational transitions associated with channel activation. Although the IP<sub>3<\/sub>-binding pocket is conserved, subtype-specific differences in IP<sub>3<\/sub>\u00a0affinity likely arise from conformational dynamics of the regulatory ARM2 domain. Comparative analyses of IP<sub>3<\/sub>R isoforms identify subtype-specific allosteric networks and domain motions that underlie differential regulation. We further define the ATP-binding site and, through mutagenesis and electrophysiology, establish the structural basis for ATP modulation of channel activity. Together, these findings reveal mechanisms of IP<sub>3<\/sub>R2 activation and subtype-specific regulation, providing a framework for understanding isoform-dependent Ca<sup>2+<\/sup>\u00a0signaling.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[53,99],"therapeutic_areas":[143],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-19710","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-assay-development-and-validation","application_areas-pores-and-toxins","therapeutic_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/19710","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=19710"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=19710"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=19710"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=19710"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=19710"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=19710"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=19710"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=19710"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=19710"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}