{"id":19217,"date":"2026-03-20T15:39:03","date_gmt":"2026-03-20T13:39:03","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=19217"},"modified":"2026-03-20T15:39:03","modified_gmt":"2026-03-20T13:39:03","slug":"2026-revisiting-vsglt-non-radioactive-characterization-of-the-vibrio-na-galactose-cotransporter-by-surfe2r-n1-solid-supported-membrane-electrophysiology","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2026-revisiting-vsglt-non-radioactive-characterization-of-the-vibrio-na-galactose-cotransporter-by-surfe2r-n1-solid-supported-membrane-electrophysiology\/","title":{"rendered":"2026 &#8211; Revisiting vSGLT: Non-Radioactive Characterization of the Vibrio Na<sup>+<\/sup>\/Galactose Cotransporter by SURFE<sup>2<\/sup>R N1 Solid-Supported Membrane Electrophysiology"},"content":{"rendered":"<p>The sodium\u2013galactose cotransporter from\u00a0<span class=\"html-italic\">Vibrio parahaemolyticus<\/span>\u00a0(vSGLT) was first cloned and functionally characterized by the laboratory of Ernest M. Wright in 2000, establishing a one-to-one Na<sup>+<\/sup>:sugar coupling stoichiometry and pioneering a bacterial model for human SGLTs. Here, we revisit vSGLT using solid-supported membrane electrophysiology on the Nanion SURFE<sup>2<\/sup>R N1, providing a modern, non-radioactive kinetic analysis of Na<sup>+<\/sup>-coupled sugar transport. Rapid transient currents were observed upon substrate application to proteoliposomes containing purified vSGLT. D-galactose elicited the largest Na<sup>+<\/sup>-dependent responses, followed by D-glucose and D-fucose, while no transport was observed in K<sup>+<\/sup>-based solutions. Apparent kinetic parameters recapitulate the overall trends observed in the original radiolabeled uptake assays, with\u00a0<span class=\"html-italic\">K<\/span><sub>m<\/sub>(Na<sup>+<\/sup>) \u2248 18 mM and\u00a0<span class=\"html-italic\">K<\/span><sub>m<\/sub>(gal) \u2248 9.8 mM. These findings validate the SURFE<sup>2<\/sup>R N1 SSM system as a quantitative, label-free method for Na<sup>+<\/sup>\u00a0symport characterization and demonstrate that vSGLT retains its canonical substrate selectivity and stoichiometry.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[53,108],"therapeutic_areas":[143],"target_categories":[60],"target_families":[375],"targets":[532],"resource_keyword":[],"class_list":["post-19217","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-assay-development-and-validation","application_areas-transporters","therapeutic_areas-assay-development-and-validation","target_categories-transporters","target_families-symporter","targets-vsglt"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/19217","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=19217"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=19217"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=19217"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=19217"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=19217"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=19217"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=19217"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=19217"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=19217"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}