{"id":17847,"date":"2025-08-08T12:01:24","date_gmt":"2025-08-08T10:01:24","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=17847"},"modified":"2025-08-08T12:01:24","modified_gmt":"2025-08-08T10:01:24","slug":"2025-cyanine-5-huwentoxin-iv-as-a-novel-imaging-probe-to-detect-hnav1-7-channel-overexpressed-in-non-small-cell-lung-cancer","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2025-cyanine-5-huwentoxin-iv-as-a-novel-imaging-probe-to-detect-hnav1-7-channel-overexpressed-in-non-small-cell-lung-cancer\/","title":{"rendered":"2025 &#8211; Cyanine 5-huwentoxin-IV as a novel imaging probe to detect hNav1.7 channel overexpressed in non-small cell lung cancer"},"content":{"rendered":"<p>Currently, tools for detecting cancer cells belonging to the non-small cell lung cancer (NSCLC) are lacking. As overexpression of human voltage-gated sodium channel subtype 1.7 (hNa<sub>v<\/sub>1.7) channels was identified as a promising marker in NSCLC, we investigated whether huwentoxin-IV (HwTx-IV), known to interact preferentially with this sodium channel subtype at nanomolar concentrations, may serve as a practical tool for detecting NSCLC. To achieve this goal, we coupled HwTx-IV to the cyanine 5 fluorophore and evaluated the potential of cyanine 5-HwTx-IV (Cy5-HwTx-IV) as an imaging agent to improve the detection of this cancer type. Increased messager ribonucleic acid (mRNA) expression levels of hNa<sub>v<\/sub>1.7 were confirmed in the A549 and H460 NSCLC cell lines, but not in the Beas-2B healthy lung cell line. Moreover, electrophysiological recordings validated the presence of functional hNa<sub>v<\/sub>1.7 in the metastatic H460 cell line, the recorded Na<sup>+<\/sup>\u00a0current in this cell line being completely abolished in the presence of nanomolar concentrations of Cy5-HwTx-IV and HwTx-IV. In contrast, functional hNa<sub>v<\/sub>1.7 was not detected in the non-metastatic A549 and healthy lung Beas-2B cell lines. Finally, confocal laser scanning microscopy allowed localisation of hNa<sub>v<\/sub>1.7 at the plasma membranes of cells labelled with Cy5-HwTx-IV. In conclusion, this work establishes Cy5-HwTx-IV as a promising tool for NSCLC imaging, thereby enhancing diagnostic precision for this cancer type.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[75],"application_areas":[50,76],"therapeutic_areas":[57],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-17847","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-publication","application_areas-ion-channels","application_areas-drug-discovery","therapeutic_areas-cancer"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/17847","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=17847"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=17847"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=17847"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=17847"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=17847"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=17847"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=17847"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=17847"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=17847"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}