{"id":17717,"date":"2025-07-14T08:48:24","date_gmt":"2025-07-14T08:48:24","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=17717"},"modified":"2025-07-14T08:50:03","modified_gmt":"2025-07-14T08:50:03","slug":"2025-exploring-new-nanopore-sensors-from-the-aerolysin-family","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2025-exploring-new-nanopore-sensors-from-the-aerolysin-family\/","title":{"rendered":"2025 &#8211; Exploring New Nanopore Sensors from the Aerolysin Family"},"content":{"rendered":"<p>Aerolysin-like proteins are a family of pore-forming toxins with potential biotechnological applications as nanopore sensors for biomolecular detection and sequencing. Despite their conserved structural fold, the low sequence identity complicates sequence alignment, limiting the understanding of their pore structure and properties. Here, the pore structures of three family members \u2013\u00a0<i>Clostridium perfringens<\/i>\u00a0epsilon toxin (ETX),\u00a0<i>Laetiporus sulphureus<\/i>\u00a0lectin (LSL), and\u00a0<i>Bacillus thuringiensis<\/i>\u00a0parasporin-2 \u2013 are analyzed and compared to aerolysin and assess their single-strand DNA (ssDNA) sensing capabilities through\u00a0<i>in silico<\/i>\u00a0methods. ETX is further characterized experimentally, revealing three distinct pore conformations, each with specific open pore currents, only one of which translocates ssDNA. Notably, ETX exhibited higher current blockage depth compared to aerolysin during ssDNA translocation, indicating a higher sensitivity for molecular sensing. The findings open new avenues for improving and diversifying nanopore capabilities in molecular sensing.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[53,99],"therapeutic_areas":[143],"target_categories":[341],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-17717","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-assay-development-and-validation","application_areas-pores-and-toxins","therapeutic_areas-assay-development-and-validation","target_categories-pores-and-toxins"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/17717","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=17717"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=17717"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=17717"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=17717"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=17717"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=17717"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=17717"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=17717"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=17717"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}