{"id":17023,"date":"2025-03-17T10:28:40","date_gmt":"2025-03-17T10:28:40","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=17023"},"modified":"2025-03-17T10:28:40","modified_gmt":"2025-03-17T10:28:40","slug":"2025-veratridine-induced-oscillations-in-nav-1-7-but-not-nav-1-5-sodium-channels-are-revealed-by-membrane-potential-sensitive-dye","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2025-veratridine-induced-oscillations-in-nav-1-7-but-not-nav-1-5-sodium-channels-are-revealed-by-membrane-potential-sensitive-dye\/","title":{"rendered":"2025 &#8211; Veratridine-Induced Oscillations in Nav 1.7 but Not Nav 1.5 Sodium Channels Are Revealed by Membrane Potential Sensitive Dye"},"content":{"rendered":"<section id=\"html-abstract\" class=\"html-abstract\">\n<div class=\"html-p\">Voltage-gated sodium channels (Na<sub>v<\/sub>s) are critical for membrane potential depolarisation in cells, with especially important roles in neuronal and cardiomyocyte membranes. Their malfunction results in a range of disorders, and they are the target of many widely used drugs. A rapid yet accurate functional assay is therefore desirable both to probe for novel active compounds and to better understand the many different Na<sub>v<\/sub> isoforms. Here, we use fluorescence to monitor Nav function: cells expressing either the cardiac Na<sub>v<\/sub> 1.5 or pain-associated Na<sub>v<\/sub> 1.7 were loaded with fluorescent membrane potential sensitive dye and then stimulated with veratridine. Cells expressing Na<sub>v<\/sub> 1.5 show a concentration-dependent slow rise and then a plateau in fluorescence. In contrast, cells expressing Na<sub>v<\/sub> 1.7 show a more rapid rise and then unexpected oscillatory behavior. Inhibition by flecainide and mexiletine demonstrates that these oscillations are Na<sub>v<\/sub>-dependent. Thus, we show that this fluorescent membrane potential dye can provide useful functional data and that we can readily distinguish between these two Na<sub>v<\/sub> isoforms because of the behavior of cells expressing them when activated by veratridine. We consider these distinct behaviors may be due to different interactions of veratridine with the different Na<sub>v<\/sub> isoforms, although more studies are needed to understand the mechanism underlying the oscillations.<\/div>\n<\/section>\n<div id=\"html-keywords\"><\/div>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[75],"application_areas":[50,53,76],"therapeutic_areas":[143],"target_categories":[61],"target_families":[332],"targets":[93,116],"resource_keyword":[],"class_list":["post-17023","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","application_areas-drug-discovery","therapeutic_areas-assay-development-and-validation","target_categories-voltage-gated-ion-channels","target_families-nav-channels","targets-nav1-5","targets-nav1-7"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/17023","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=17023"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=17023"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=17023"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=17023"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=17023"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=17023"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=17023"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=17023"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=17023"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}