{"id":14749,"date":"2024-07-04T08:52:04","date_gmt":"2024-07-04T08:52:04","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=14749"},"modified":"2024-07-04T08:52:04","modified_gmt":"2024-07-04T08:52:04","slug":"2024-variations-in-candidalysin-amino-acid-sequence-influence-toxicity-and-host-responses","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2024-variations-in-candidalysin-amino-acid-sequence-influence-toxicity-and-host-responses\/","title":{"rendered":"2024 &#8211; Variations in candidalysin amino acid sequence influence toxicity and host responses"},"content":{"rendered":"<p><i>Candida albicans<\/i>\u00a0causes millions of mucosal infections in humans annually. Hyphal overgrowth on mucosal surfaces is frequently associated with tissue damage caused by candidalysin, a secreted peptide toxin that destabilizes the plasma membrane of host cells thereby promoting disease and immunopathology. Candidalysin was first identified in\u00a0<i>C. albicans<\/i>\u00a0strain SC5314, but recent investigations have revealed candidalysin \u201cvariants\u201d of differing amino acid sequence in isolates of\u00a0<i>C. albicans<\/i>, and the related species\u00a0<i>C. dubliniensis<\/i>, and\u00a0<i>C tropicalis<\/i>, suggesting that sequence variation among candidalysins may be widespread in natural populations of these\u00a0<i>Candida<\/i>\u00a0species. Here, we analyzed\u00a0<i>ECE1<\/i>\u00a0gene sequences from 182\u00a0<i>C<\/i>.\u00a0<i>albicans<\/i>\u00a0isolates, 10\u00a0<i>C<\/i>.\u00a0<i>dubliniensis<\/i>\u00a0isolates, and 78\u00a0<i>C<\/i>.\u00a0<i>tropicalis<\/i>\u00a0isolates and identified 10, 3, and 2 candidalysin variants in these species, respectively. Application of candidalysin variants to epithelial cells revealed differences in the ability to cause cellular damage, changes in metabolic activity, calcium influx, MAPK signalling, and cytokine secretion, while biophysical analyses indicated that variants exhibited differences in their ability to interact with and permeabilize a membrane. This study identifies candidalysin variants with differences in biological activity that are present in medically relevant\u00a0<i>Candida<\/i>\u00a0species.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[98],"resource_type":[75],"application_areas":[53,99],"therapeutic_areas":[143,290],"target_categories":[341],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-14749","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-16-tc","resource_type-publication","application_areas-assay-development-and-validation","application_areas-pores-and-toxins","therapeutic_areas-assay-development-and-validation","therapeutic_areas-toxicology","target_categories-pores-and-toxins"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/14749","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=14749"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=14749"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=14749"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=14749"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=14749"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=14749"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=14749"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=14749"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=14749"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}