{"id":14439,"date":"2024-05-23T07:34:58","date_gmt":"2024-05-23T07:34:58","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=14439"},"modified":"2024-05-23T07:34:58","modified_gmt":"2024-05-23T07:34:58","slug":"2024-an-experimental-investigation-of-rundown-of-the-l-type-calcium-current","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2024-an-experimental-investigation-of-rundown-of-the-l-type-calcium-current\/","title":{"rendered":"2024 &#8211; An experimental investigation of rundown of the L-type calcium current"},"content":{"rendered":"<p>Background<br \/>\nL-type calcium channels (LCCs) are multi-protein macro-molecular ion channel complexes that are involved in several critical functions in cardiac, skeletal, neuronal, smooth muscle, and endocrine cells. Like other ion channels, LCCs can be selectively over-expressed in a host cell line and studied using voltage-clamp patch-clamp experiments. However, L-type calcium current (ICaL) recordings commonly exhibit a reduction in current magnitude over time, commonly termed \u2018rundown\u2019. Previous studies have shown the effect of phosphorylation on rundown, here we provide evidence that accumulation of Ca<sup>2+<\/sup> inside the cell also contributes towards ICaL rundown.<\/p>\n<p>Methods<br \/>\nWe generated experimental conditions that should promote the accumulation of sub-membrane Ca<sup>2+<\/sup> in a CHO expression system, by increasing calcium import or decreasing export. These interventions took the form of: a decrease in inter-pulse duration between sweeps, block of the sodium-calcium exchanger, and increased temperature.<\/p>\n<p>Results<br \/>\nOn average, we found that current reduced to 63% of its initial value within 325 seconds. This reduction of current with time was found to follow two main patterns: linear or saturating decay. Additionally, current magnitude in some cells increased before stabilising or decaying.<\/p>\n<p>Conclusions<br \/>\nThis study shows that the rundown of ICaL in patch-clamp experiments can be reduced by modifying the experimental conditions, and implies that reduced accumulation of Ca<sup>2+<\/sup> inside the cell membrane reduces calcium-dependent inactivation of ICaL.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61],"target_families":[367],"targets":[91],"resource_keyword":[],"class_list":["post-14439","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-publication","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_families-cacc-channels","targets-cav1-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/14439","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=14439"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=14439"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=14439"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=14439"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=14439"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=14439"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=14439"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=14439"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=14439"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}