{"id":14266,"date":"2024-04-25T15:27:07","date_gmt":"2024-04-25T15:27:07","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=14266"},"modified":"2024-04-25T15:27:07","modified_gmt":"2024-04-25T15:27:07","slug":"2024-yeed-is-an-essential-partner-for-yeee-mediated-thiosulfate-uptake-in-bacteria-and-regulates-thiosulfate-ion-decomposition","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2024-yeed-is-an-essential-partner-for-yeee-mediated-thiosulfate-uptake-in-bacteria-and-regulates-thiosulfate-ion-decomposition\/","title":{"rendered":"2024 &#8211; YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition"},"content":{"rendered":"<p>Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in\u00a0<em>Escherichia coli<\/em>\u00a0was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via\u00a0<em>Spirochaeta thermophila<\/em>\u00a0YeeE in vitro and characterized\u00a0<em>E<\/em>.\u00a0<em>coli<\/em>\u00a0YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that\u00a0<em>S<\/em>.\u00a0<em>thermophila<\/em>\u00a0YeeD possesses thiosulfate decomposition activity and that a conserved cysteine in YeeD was modified to several forms in the presence of thiosulfate. Finally, the crystal structures of\u00a0<em>S<\/em>.\u00a0<em>thermophila<\/em>\u00a0YeeE-YeeD fusion proteins at 3.34-\u00c5 and 2.60-\u00c5 resolutions revealed their interactions. The association was evaluated by a binding assay using purified\u00a0<em>S<\/em>.\u00a0<em>thermophila<\/em>\u00a0YeeE and YeeD. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[53,108],"therapeutic_areas":[143],"target_categories":[60],"target_families":[349],"targets":[469],"resource_keyword":[],"class_list":["post-14266","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-assay-development-and-validation","application_areas-transporters","therapeutic_areas-assay-development-and-validation","target_categories-transporters","target_families-prokaryotic-pores","targets-yeee"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/14266","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=14266"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=14266"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=14266"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=14266"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=14266"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=14266"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=14266"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=14266"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=14266"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}