{"id":14215,"date":"2024-04-18T07:42:59","date_gmt":"2024-04-18T07:42:59","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=14215"},"modified":"2024-04-18T07:42:59","modified_gmt":"2024-04-18T07:42:59","slug":"2024-automated-patch-clamp-for-the-detection-of-tetrodotoxin-in-pufferfish-samples","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2024-automated-patch-clamp-for-the-detection-of-tetrodotoxin-in-pufferfish-samples\/","title":{"rendered":"2024 &#8211; Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples"},"content":{"rendered":"<p>Tetrodotoxin (TTX) is a marine toxin responsible for many intoxications around the world. Its presence in some pufferfish species and, as recently reported, in shellfish, poses a serious health concern. Although TTX is not routinely monitored, there is a need for fast, sensitive, reliable, and simple methods for its detection and quantification. In this work, we describe the use of an automated patch clamp (APC) system with Neuro-2a cells for the determination of TTX contents in pufferfish samples. The cells showed an IC<sub>50<\/sub>\u00a0of 6.4 nM for TTX and were not affected by the presence of muscle, skin, liver, and gonad tissues of a\u00a0<span class=\"html-italic\">Sphoeroides pachygaster<\/span>\u00a0specimen (TTX-free) when analysed at 10 mg\/mL. The LOD achieved with this technique was 0.05 mg TTX equiv.\/kg, which is far below the Japanese regulatory limit of 2 mg TTX equiv.\/kg. The APC system was applied to the analysis of extracts of a\u00a0<span class=\"html-italic\">Lagocephalus sceleratus<\/span>\u00a0specimen, showing TTX contents that followed the trend of gonads &gt; liver &gt; skin &gt; muscle. The APC system, providing an in vitro toxicological approach, offers the advantages of being sensitive, rapid, and reliable for the detection of TTX-like compounds in seafood.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[75],"application_areas":[50,53],"therapeutic_areas":[143,290],"target_categories":[61],"target_families":[332],"targets":[],"resource_keyword":[],"class_list":["post-14215","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","therapeutic_areas-assay-development-and-validation","therapeutic_areas-toxicology","target_categories-voltage-gated-ion-channels","target_families-nav-channels"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/14215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=14215"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=14215"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=14215"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=14215"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=14215"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=14215"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=14215"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=14215"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=14215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}