{"id":13417,"date":"2023-05-17T08:15:32","date_gmt":"2023-05-17T08:15:32","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=13417"},"modified":"2024-01-17T08:15:58","modified_gmt":"2024-01-17T08:15:58","slug":"2023-amphipathic-peptide-phospholipid-nanofibers-kinetics-of-fiber-formation-and-molecular-transfer-between-assemblies","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2023-amphipathic-peptide-phospholipid-nanofibers-kinetics-of-fiber-formation-and-molecular-transfer-between-assemblies\/","title":{"rendered":"2023 &#8211; Amphipathic peptide\u2013phospholipid nanofibers: Kinetics of fiber formation and molecular transfer between assemblies"},"content":{"rendered":"<p>Understanding the kinetics of nano-assembly formation is important to elucidate the\u00a0biological processes\u00a0involved and develop novel\u00a0nanomaterials\u00a0with biological functions. In the present study, we report the kinetic mechanisms of\u00a0nanofiber\u00a0formation from a mixture of\u00a0phospholipids\u00a0and the amphipathic peptide 18A[A11C], carrying cysteine substitution of the\u00a0apolipoprotein\u00a0A-I-derived peptide 18A at residue 11. 18A[A11C] with acetylated N-terminus and amidated C-terminus can associate with\u00a0phosphatidylcholine\u00a0to form\u00a0fibrous\u00a0aggregates at neutral pH and lipid-to-peptide molar ratio of \u223c1, although the reaction pathways of self-assembly remain unclear. Here, the peptide was added to giant 1-palmitoyl-2-oleoyl\u00a0phosphatidylcholine\u00a0vesicles to monitor\u00a0nanofiber\u00a0formation under\u00a0fluorescence microscopy. The peptide initially solubilized the\u00a0lipid vesicles\u00a0into particles smaller than the resolution of optical microscope, and\u00a0fibrous\u00a0aggregates appeared subsequently.\u00a0Transmission electron microscopy\u00a0and\u00a0dynamic light scattering\u00a0analyses revealed that the vesicle-solubilized particles were spherical or circular, measuring \u223c10\u201320\u00a0nm in diameter. The rate of nanofiber formation of 18A with 1,2-dipalmitoyl phosphatidylcholine from the particles was proportional to the square of lipid\u2013peptide concentration in the system, suggesting that the association of particles, accompanied by conformational changes, was the rate-limiting step. Moreover, molecules in the nanofibers could be transferred between aggregates faster than those in the\u00a0lipid\u00a0vesicles. These findings provide useful information for the development and control of nano-assembling structures using peptides and\u00a0phospholipids.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[129],"resource_type":[75],"application_areas":[53],"therapeutic_areas":[143],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-13417","resource_library","type-resource_library","status-publish","hentry","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-assay-development-and-validation","therapeutic_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/13417","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=13417"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=13417"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=13417"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=13417"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=13417"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=13417"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=13417"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=13417"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=13417"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}