{"id":13398,"date":"2023-12-19T17:17:13","date_gmt":"2023-12-19T17:17:13","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=13398"},"modified":"2024-01-11T17:17:38","modified_gmt":"2024-01-11T17:17:38","slug":"2023-deep-learning-assisted-single-molecule-detection-of-protein-post-translational-modifications-with-a-biological-nanopore","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2023-deep-learning-assisted-single-molecule-detection-of-protein-post-translational-modifications-with-a-biological-nanopore\/","title":{"rendered":"2023 &#8211; Deep Learning-Assisted Single-Molecule Detection of Protein Post-translational Modifications with a Biological Nanopore"},"content":{"rendered":"<p>Protein post-translational modifications (PTMs) play a crucial role in countless biological processes, profoundly modulating protein properties on both spatial and temporal scales. Protein PTMs have also emerged as reliable biomarkers for several diseases. However, only a handful of techniques are available to accurately measure their levels, capture their complexity at a single molecule level, and characterize their multifaceted roles in health and disease. Nanopore sensing provides high sensitivity for the detection of low-abundance proteins, holding the potential to impact single-molecule proteomics and PTM detection, in particular. Here, we demonstrate the ability of a biological nanopore, the pore-forming toxin aerolysin, to detect and distinguish \u03b1-synuclein-derived peptides bearing single or multiple PTMs, namely, phosphorylation, nitration, and oxidation occurring at different positions and in various combinations. The characteristic current signatures of the \u03b1-synuclein peptide and its PTM variants could be confidently identified by using a deep learning model for signal processing. We further demonstrate that this framework can quantify \u03b1-synuclein peptides at picomolar concentrations and detect the C-terminal peptides generated by digestion of full-length \u03b1-synuclein. Collectively, our work highlights the advantage of using nanopores as a tool for simultaneous detection of multiple PTMs and facilitates their use in biomarker discovery and diagnostics.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[53,99],"therapeutic_areas":[143],"target_categories":[341],"target_families":[],"targets":[242,259],"resource_keyword":[],"class_list":["post-13398","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-assay-development-and-validation","application_areas-pores-and-toxins","therapeutic_areas-assay-development-and-validation","target_categories-pores-and-toxins","targets-dna-nanopores","targets-aerolysin"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/13398","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=13398"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=13398"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=13398"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=13398"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=13398"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=13398"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=13398"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=13398"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=13398"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}