{"id":12065,"date":"2023-07-11T10:15:02","date_gmt":"2023-07-11T10:15:02","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=12065"},"modified":"2023-07-11T10:15:02","modified_gmt":"2023-07-11T10:15:02","slug":"teer-monitoring-cell-attachment-and-tight-junctions-in-the-same-assay","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/teer-monitoring-cell-attachment-and-tight-junctions-in-the-same-assay\/","title":{"rendered":"TEER &#8211; &#8220;Monitoring cell \u2013 attachment and tight junctions in the same assay&#8221;"},"content":{"rendered":"<p>The numerous different cell types in the human body are greatly specialized and often require a conjunctive action of a population of cells, for example in tissues. Cells are interconnected via cell junctions, multiprotein complexes found in the cell membrane of animal cells, and such cell junctions allow for a mechanical, chemical or electrical transmission of signals. These junctions can be subdivided into (I) tight junctions, (II) anchoring junctions or (III) gap junctions. Defects in cell\u2013cell junctions give rise to a wide range of tissue abnormalities that disrupt homeostasis and are common in genetic abnormalities and cancers (1). The so-called tight junctions form the barrier in endothelial and epithelial cells. Classical transepithelial electrical resistance measurements are performed using microelectrodes, where trans- and para-cellular conductivities can be calculated (2).<br \/>\nHere the cells are grown on a porous filter membrane which is placed between two fluid compartments. Flux of solutes from one compartment to the other must pass the interfacial cell<br \/>\nlayer then, and this is determined by the functional properties of the tight junctions.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[419],"resource_type":[90],"application_areas":[50,53,76,77],"therapeutic_areas":[109,143],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-12065","resource_library","type-resource_library","status-publish","hentry","products_tags-atlaz","resource_type-application-note","application_areas-ion-channels","application_areas-assay-development-and-validation","application_areas-drug-discovery","application_areas-cell-ipscs-profiling","therapeutic_areas-respiratory-diseases","therapeutic_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/12065","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=12065"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=12065"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=12065"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=12065"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=12065"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=12065"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=12065"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=12065"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=12065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}