{"id":11612,"date":"2023-07-03T08:31:03","date_gmt":"2023-07-03T08:31:03","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=11612"},"modified":"2024-01-11T13:07:23","modified_gmt":"2024-01-11T13:07:23","slug":"2023-electrophysiological-and-calcium-handling-development-during-long-term-culture-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2023-electrophysiological-and-calcium-handling-development-during-long-term-culture-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes\/","title":{"rendered":"2023 &#8211; Electrophysiological and calcium-handling development during long-term culture of human-induced pluripotent stem cell-derived cardiomyocytes"},"content":{"rendered":"<p>Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are increasingly used for personalised medicine and preclinical cardiotoxicity testing. Reports on hiPSC-CM commonly describe heterogenous functional readouts and underdeveloped or immature phenotypical properties. Cost-effective, fully defined monolayer culture is approaching mainstream adoption; however, the optimal age at which to utilise hiPSC-CM is unknown. In this study, we identify, track and model the dynamic developmental behaviour of key ionic currents and Ca<sup>2+<\/sup>-handling properties in hiPSC-CM over long-term culture (30\u201380\u00a0days). hiPSC-CMs\u2009&gt;\u200950\u00a0days post differentiation show significantly larger\u00a0<i>I<\/i><sub>Ca,L<\/sub>\u00a0density along with an increased\u00a0<i>I<\/i><sub>Ca,L<\/sub>-triggered Ca<sup>2+<\/sup>-transient.\u00a0<i>I<\/i><sub>Na<\/sub>\u00a0and\u00a0<i>I<\/i><sub><i>K<\/i>1<\/sub>\u00a0densities significantly increase in late-stage cells, contributing to increased upstroke velocity and reduced action potential duration, respectively. Importantly, our in silico model of hiPSC-CM electrophysiological age dependence confirmed\u00a0<i>I<\/i><sub><i>K<\/i>1<\/sub>\u00a0as the key ionic determinant of action potential shortening in older cells. We have made this model available through an open source software interface that easily allows users to simulate hiPSC-CM electrophysiology and Ca<sup>2+<\/sup>-handling and select the appropriate age range for their parameter of interest. This tool, together with the insights from our comprehensive experimental characterisation, could be useful in future optimisation of the culture-to-characterisation pipeline in the field of hiPSC-CM research.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[75],"application_areas":[53,77],"therapeutic_areas":[54,143],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-11612","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-publication","application_areas-assay-development-and-validation","application_areas-cell-ipscs-profiling","therapeutic_areas-cardiac-safety-cipa","therapeutic_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/11612","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=11612"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=11612"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=11612"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=11612"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=11612"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=11612"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=11612"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=11612"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=11612"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}