The Orbit mini is a miniaturized bilayer workstation for recording from 4 bilayers simultaneously.<\/p>\n
Watch how to prepare membranes for the Orbit mini:<\/p>\n
1. Take e.g. a 10 \u03bcl Eppendorf pipette (small white tip)<\/p>\n
2. Cover the tip with lipids by dipping it into the lipid stock solution without aspirating any of it. Due to capillary forces a small amount of lipid solution will remain in the pipette tip – squeeze out any of these residues from the pipette tip<\/p>\n
3. Place the tip on the chip\u2019s surface close to a cavity, dispense a small air bubble (2-3 \u03bcl) over the cavity and aspirate it back. This results in the formation of a lipid bilayer which can be monitored by following the change in the current response: the signal should not go into saturation anymore and exhibit capacitive properties as shown on the right<\/p>\n
Repeat step 3 on all cavities.<\/p>\n","protected":false},"excerpt":{"rendered":"
The Orbit mini is a miniaturized bilayer workstation for recording from 4 bilayers simultaneously. Watch how t […]<\/p>\n","protected":false},"featured_media":8850,"template":"","news_categories":[],"class_list":["post-9894","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news\/9894","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/news"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media\/8850"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=9894"}],"wp:term":[{"taxonomy":"news_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news_categories?post=9894"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}