{"id":17927,"date":"2025-08-27T14:36:46","date_gmt":"2025-08-27T12:36:46","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=news&#038;p=17927"},"modified":"2025-08-27T17:18:06","modified_gmt":"2025-08-27T15:18:06","slug":"nanodiscs-preserve-trpc3-channel-activity-for-structural-and-functional-studies","status":"publish","type":"news","link":"https:\/\/nanion.com\/ja\/news\/nanodiscs-preserve-trpc3-channel-activity-for-structural-and-functional-studies\/","title":{"rendered":"Nanodiscs preserve TRPC3 channel activity for structural and functional studies"},"content":{"rendered":"<p data-start=\"198\" data-end=\"532\">The canonical transient receptor potential channel 3<strong data-start=\"198\" data-end=\"262\"> (TRPC3)<\/strong> plays key roles in GPCR signaling and is linked to several diseases, including cardiac arrhythmias, cancer, and neurodegeneration. Despite advances in cryo-EM, existing structures show TRPC3 only in its closed state, leaving the open-pore conformation unresolved.<\/p>\n<p data-start=\"198\" data-end=\"532\">Importantly, TRPC3 is <strong data-start=\"452\" data-end=\"497\">highly sensitive to its lipid environment<\/strong>. Lipids such as diacylglycerol (DAG), cholesterol, and PIP\u2082 directly influence its gating and stability. When removed from these native lipid interactions, TRPC3 often loses function or remains closed \u2014 a major barrier for structural studies.<\/p>\n<p data-start=\"534\" data-end=\"979\">In a new study, researchers from the University of Graz and collaborators in Austria and France addressed this challenge by testing different <strong data-start=\"680\" data-end=\"696\">cell systems<\/strong> and <strong data-start=\"701\" data-end=\"722\">extraction agents<\/strong> to purify functional TRPC3 while preserving its lipid environment. They compared adherent HEK293, Expi293F, and <em data-start=\"835\" data-end=\"847\">K. phaffii<\/em> yeast cells, evaluating traditional detergents (DDM, LMNG, FOS-choline) and nanodisc-forming agents (DIBMA, Carboxy-DIBMA, DDDG).<\/p>\n<p>&nbsp;<\/p>\n<p data-start=\"981\" data-end=\"1000\"><strong data-start=\"981\" data-end=\"998\">Key findings:<\/strong><\/p>\n<ul data-start=\"1001\" data-end=\"1425\">\n<li data-start=\"1001\" data-end=\"1060\">\n<p data-start=\"1003\" data-end=\"1060\">Expi293F cells produced the highest yield of TRPC3.<\/p>\n<\/li>\n<li data-start=\"1061\" data-end=\"1149\">\n<p data-start=\"1063\" data-end=\"1149\">DDDG and DDM best preserved structure and function under native-like conditions.<\/p>\n<\/li>\n<li data-start=\"1150\" data-end=\"1256\">\n<p data-start=\"1152\" data-end=\"1256\">Negative-stain EM confirmed the purified protein retained its tetrameric \u201cflower-shaped\u201d assembly.<\/p>\n<\/li>\n<li data-start=\"1150\" data-end=\"1256\">\n<p data-start=\"1230\" data-end=\"1293\">Electrophysiology showed that purified and reconstituted TRPC3 channels remained functional, with conductance and gating behavior comparable to those in cells (bilayer recordings performed using the Orbit mini).<\/p>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p data-start=\"1427\" data-end=\"1723\">These results demonstrate that TRPC3 can be stably purified in nanodiscs or mild detergents without losing activity. This provides robust conditions for future cryo-EM studies to finally capture TRPC3 in its open state, deepening our understanding of its function and therapeutic potential.<\/p>\n<p data-start=\"2182\" data-end=\"2185\">\u2014<\/p>\n<p data-start=\"2187\" data-end=\"2347\">\ud83d\udcd6 Read the full article: <a href=\"https:\/\/www.nature.com\/articles\/s41598-025-13218-6\" target=\"_blank\" rel=\"noopener\">Utilizing native nanodiscs to isolate active TRPC3 channels and expand structural analysis capabilities (Scientific Reports, 2025)<\/a><\/p>\n<p data-start=\"2349\" data-end=\"2432\">Learn more about the <a href=\"\/products\/orbit-mini\/\">Orbit mini<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The canonical transient receptor potential channel 3 (TRPC3) plays key roles in GPCR signaling and is linked t [&hellip;]<\/p>\n","protected":false},"featured_media":17928,"template":"","news_categories":[528,15],"class_list":["post-17927","news","type-news","status-publish","has-post-thumbnail","hentry","news_categories-front-page-news","news_categories-blogs"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news\/17927","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/news"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media\/17928"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=17927"}],"wp:term":[{"taxonomy":"news_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news_categories?post=17927"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}