{"id":17134,"date":"2025-04-10T11:13:35","date_gmt":"2025-04-10T11:13:35","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=news&#038;p=17134"},"modified":"2025-04-10T11:13:35","modified_gmt":"2025-04-10T11:13:35","slug":"comprehensive-maturation-of-ipsc-cms-through-metabolic-structural-and-electrical-interventions","status":"publish","type":"news","link":"https:\/\/nanion.com\/ja\/news\/comprehensive-maturation-of-ipsc-cms-through-metabolic-structural-and-electrical-interventions\/","title":{"rendered":"Combined approach to improve iPSC-CM maturation"},"content":{"rendered":"<p class=\"\" data-start=\"229\" data-end=\"624\">Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are a widely used platform in cardiac research. They offer a patient-specific model for investigating disease mechanisms, cardiotoxicity, and drug responses. However, their structural and functional immaturity remains a significant limitation, particularly for applications requiring adult-like electrophysiological behavior.<\/p>\n<p class=\"\" data-start=\"626\" data-end=\"951\">A recent study by Wener Li and colleagues (Technische Universit\u00e4t Dresden) explored how combining metabolic, structural, and electrical treatments could promote iPSC-CM maturation over a 42-day protocol:<\/p>\n<ul>\n<li data-start=\"1018\" data-end=\"1118\">Metabolic maturation medium (MM): Supplemented with fatty acids and higher calcium.<\/li>\n<li data-start=\"1121\" data-end=\"1225\">Nanopatterning (NP): Used to promote cell alignment.<\/li>\n<li>Electrostimulation (ES): Applied at 2 Hz to mimic physiological heart rhythms.<\/li>\n<\/ul>\n<p data-start=\"1267\" data-end=\"1293\">What did they observe?<\/p>\n<ul>\n<li data-start=\"1213\" data-end=\"1340\"><span style=\"text-decoration: underline;\">Improved structural organization<\/span> with aligned sarcomeres and enhanced Cx43 membrane localization in the MM+NP+ES condition.<\/li>\n<li data-start=\"1343\" data-end=\"1508\"><span style=\"text-decoration: underline;\">Mature electrophysiological signatures<\/span>, including \u201cnotch-and-dome\u201d action potentials, hyperpolarized resting membrane potential, and increased upstroke velocity.<\/li>\n<li data-start=\"1511\" data-end=\"1623\"><span style=\"text-decoration: underline;\">Enhanced drug response<\/span>, with more predictable effects of verapamil and E-4031 compared to immature iPSC-CMs.<\/li>\n<li data-start=\"1626\" data-end=\"1794\"><span style=\"text-decoration: underline;\">Transcriptomic insights<\/span> suggesting mitochondrial activation (via TFAM, HMCES) and downregulation of MAPK\/PI3K-AKT pathways linked to polyploidy and differentiation.<\/li>\n<\/ul>\n<p class=\"\" data-start=\"2319\" data-end=\"2581\">To assess ion channel function, selected current measurements (I<sub>Na<\/sub>, I<sub>K1<\/sub>, I<sub>to<\/sub>, I<sub>Ca-L<\/sub>) were obtained using automated patch clamp (Patchliner). This approach provided reproducible single-cell data across different maturation conditions, supporting the interpretation of functional outcomes.<\/p>\n<p class=\"\" data-start=\"2598\" data-end=\"2827\">This study highlights the benefits of combining metabolic, structural, and electrical signal to help iPSC-CMs behave more like adult heart cells. The findings are especially relevant for improving drug testing and disease modeling.<\/p>\n<p class=\"\" data-start=\"2829\" data-end=\"2830\">\u2014<\/p>\n<p class=\"\" data-start=\"2832\" data-end=\"3031\">\ud83d\udd17 Read the full article:<br data-start=\"2861\" data-end=\"2864\" \/><a href=\"https:\/\/naniontech.sharepoint.com\/sites\/Nanion-InternalCommunication\">Comprehensive promotion of iPSC-CM maturation by integrating metabolic medium with nanopatterning and electrostimulation<\/a><\/p>\n<p class=\"\" data-start=\"3033\" data-end=\"3162\">\ud83d\udcca Learn more about Nanion\u2019s automated patch clamp systems:<br data-start=\"3096\" data-end=\"3099\" \/><a class=\"\" href=\"\/product-categories\/automated-patch-clamp\/\" target=\"_new\" rel=\"noopener\" data-start=\"3099\" data-end=\"3162\">https:\/\/nanion.com\/product-categories\/automated-patch-clamp\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are a widely used platform in cardiac re [&hellip;]<\/p>\n","protected":false},"featured_media":17139,"template":"","news_categories":[528,15],"class_list":["post-17134","news","type-news","status-publish","has-post-thumbnail","hentry","news_categories-front-page-news","news_categories-blogs"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news\/17134","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/news"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media\/17139"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=17134"}],"wp:term":[{"taxonomy":"news_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/news_categories?post=17134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}