In the quest to produce artificial cells, one key challenge that remains to besolved is the recreation of a complex cellular membrane. Among the existingmodels, giant unilamellar vesicles (GUVs) are particularly interesting due totheir intrinsic compartmentalisation ability and their resemblance in size andshape to eukaryotic cells. Many techniques have been developed to produceGUVs all having inherent advantages and disadvantages. Here, the authorsshow that fluorinated silica nanoparticles (FNPs) used to form Pickeringemulsions in a fluorinated oil can destabilise lipid nanosystems to templatethe formation of GUVs. This technique enables GUV production across abroad spectrum of buffer conditions, while preventing the leakage of theencapsulated components into the oil phase. Furthermore, a simplecentrifugation process is sufficient for the release of the emulsion-trappedGUVs, bypassing the need to use emulsion-destabilising chemicals. Withfluorescent FNPs and transmission electron microscopy, the authors confirmthat FNPs are efficiently removed, producing contaminant-free GUVs. Furtherexperiments assessing the lateral diffusion of lipids and unilamellarity of theGUVs demonstrate that they are comparable to GUVs produced viaelectroformation. Finally, the ability of incorporating transmembrane proteinsis demonstrated, highlighting the potential of this method for the productionof GUVs for artificial cell applications.